Prestained Protein Standards (2024)

Overview

Free Protein Standards Selection Magnet

Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

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Prestained Protein Standards (3)

Free Protein Standards Temporary Tattoo

Two students celebrated their love of science with Precision Plus Protein Kaleidoscope Ladder tattoos. Read their story and request a free set of matching (temporary) tattoos here.

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Precision Plus Protein™ Prestained Standards are available in Dual Color, All Blue, Kaleidoscope™, and Dual Xtra options. All four standards have the same gel migration patterns, with three high-intensity reference bands (25, 50, and 75 kD).

New and Improved Dual Color Standards:

  • Brighter for easier identification of target protein
  • Sharper for more accurate molecular weight estimation
  • Stronger band intensity throughout blot development

The overall brightness of Precision Plus Dual Color Standards has been increased for visual robustness throughout the entire protein electrophoresis and western blotting workflow. Improved Dual Color Standards are now brighter than the competition on both gels and blots.

Dual Xtra Standards

Dual Xtra Standards consist of 12 recombinant proteins between 2 and 250 kD; all others consist of 10 recombinant proteins between 10 and 250 kD (note that the 2 kD band is not visible on fluorescent blots).

Kaleidoscope Standards

Kaleidoscope Standards are a mixture of ten recombinant proteins in four colors (blue, pink, yellow, and green; 10–250 kD), enabling easy gel and blot orientation via the multicolor pattern.

Features and Benefits of Precision Plus Protein Prestained Standards

  • MW markers confirmed by mass spectrometry
  • Strong linear relationship between markers' log molecular weights and relative migration distances
  • Prestained bands for easy visual monitoring of electrophoresis
  • Fluorescent dyes for imaging on gels blots

Applications of Precision Plus Protein Prestained Standards

  • Accurate molecular weight estimation
  • Monitoring of electrophoresis and transfer efficiency

More Information

Fluorescence Properties of Precision Plus Protein Prestained Standards
Fluorescent Bands
Visible Band ColorExcitation ColorAll BlueDual ColorDual Color ExtraKaleidoscope
Blue635 nm laser
(Red LED)
10–250 kD10–20, 37, 50, 100–250 kD5–20, 37, 50, 100–250 kD15, 20, 50, 100–250 kD
Pink532 nm laser
(Green LED)
25, 75 kD25, 75 kD25, 75 kD
Green635 nm laser
(Red LED)
37 kD
Yellow488 nm laser
(Blue LED)
10 kD

See Bulletin 5685 and Bulletin 5723 for more details on the fluorescence properties of Precision Plus Protein Prestained Standards.

Related Technologies

  • Introduction to Polyacrylamide Gels
  • Buffer Systems and Gel Chemistries
  • Protein Electrophoresis Reagent Selection and Preparation

Related Products

  • Criterion Blotter
  • Sample Loading Buffers and Reagents
  • Criterion™ Cell
  • Running Buffers and Reagents
  • PowerPac Universal Power Supply

Specifications

MW size range

2–250 kD

Stain type

Markers consist of highly purified recombinant proteins covalently stained with multicolored dyes

Volume

500 µl (value pack: 2,500 µl)

Total number of applications

50 (value pack: 250 applications)

Loading buffer

30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3

Shelf life

Store for 6 months at 4°C or –20°C until expiration date (Kaleidescope: 12 months at –20°C)

Contents

1 x 500 µl tube, instruction sheet, and standards migration sticker

Recommended loading volume

Electrophoresis: 10 μl
Blotting: 7 μl

Ordering

items

Use the filters below to refine results!

Image

Prestained Protein Standards (4)

(Discontinued) Precision Plus Protein Dual Color Standards, 500 µl

1610374

500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications

Image

Prestained Protein Standards (5)

(Discontinued) Precision Plus Protein™ Dual Color Standards, trial size

1610374S

50 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 5 applications

Image

Prestained Protein Standards (6)

(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml

1610394

2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

Image

Prestained Protein Standards (7)

(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards

1610373

500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications

Image

Prestained Protein Standards (8)

(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards, value pack

1610393

2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications

Image

Prestained Protein Standards (9)

(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards

1610375

500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications

Image

Prestained Protein Standards (10)

(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack

1610395

2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

Image

Prestained Protein Standards (11)

(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards

1610377

500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications

Image

Prestained Protein Standards (12)

(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack

1610397

2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications

Suggested

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Prestained Protein Standards (13)

(Discontinued) 2x Laemmli Sample Buffer

1610737

30 ml, premixed protein sample buffer for SDS-PAGE

Image

Prestained Protein Standards (14)

(Discontinued) 2-Mercaptoethanol

1610710

25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

Image

Prestained Protein Standards (15)

(Discontinued) XT Sample Buffer

1610791

10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels

Image

Prestained Protein Standards (16)

(Discontinued) XT Reducing Agent

1610792

1 ml, 20x reducing agent for use with all Criterion XT gels

Image

Prestained Protein Standards (17)

(Discontinued) Tricine Sample Buffer for Protein Gels, 30 ml

1610739

30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250

Image

Prestained Protein Standards (18)

(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml

1610363

1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

Image

Prestained Protein Standards (19)

(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl

1610376

250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

Image

Prestained Protein Standards (20)

(Discontinued) Precision Plus Protein™ Standard Plugs, unstained, Strep-tagged, recombinant

1610378

Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands

Documents

Login Required

Number Description Options

4110025

Instruction Manual, Dual Color Precision Plus Protein™ Standards, Rev H

10018393

Instruction Manual, Precision Plus Protein™ Dual Xtra Standards, Rev D

4110182

Instruction Manual, Precision Plus Protein™ Kaleidoscope Standards, Rev G

4110024

Instruction Manual, All Blue Precision Plus Protein Standards, Rev D

3092

Precision Plus Protein Standards Flier, Rev B

6536

Precision Plus Protein™ Dual Color Standard Flier, Rev B

5957

Precision Plus Protein Dual Xtra Standards Product Information Sheet, Rev B

2800

Precision Plus Protein Dual Color Standards Competitive Card, Rev C

3093

Precision Plus Protein Standards Competitive Comparison Card, Rev B

3118

Protein Standards for Electrophoresis and Blotting Brochure, Rev D

2998

Protein Standards Application Guide, Rev G

2414

The Little Book of Standards, Rev D

2895

Protein Blotting Guide, Ver C

6040

Electrophoresis Guide, Interactive PDF, Rev C

6533

Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A

3144

Using Precision Plus Protein Standards to Determine Molecular Weight, Rev B

3133

Molecular Weight Determination by SDS-PAGE, Rev B

5956

Precision Plus Protein Dual Xtra Standards — New Protein Standards with an Extended Range from 2 to 250 kD, Rev B

Prestained Protein Standards (2024)
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